SCO cells do not show a constricted morphology under normoglycemic conditions.

(A, B) Tile-scanning TEM. Under normoglycemic conditions, SCO cells did not exhibit structural changes, were normally polarized, and had dilated ER cisternae (A, ER area), and apical areas with blebs containing secretory granules, microvilli and cilia (B, C, D, black arrows). MVB, most of them containing few vesicles (E), were also detected. Scale bar: A, 10 μm; B, ER and nuclear areas, 2 μm; C to E, 0.2 μm. N = 3. BB, basal body; BV, blood vessel; ER, endoplasmic reticulum; MVB, multivesicular body; SCO, subcommissural organ; TEM, transmission electron microscopy. (TIF)