αSyn C-terminal peptides disrupt the interaction between vaccine-induced antibodies and αSyn.

Adult mice (n = 7) were administered with Ct-αSyn complex vaccine via intraperitoneal injection four times at 2-week intervals. Sera were collected from tail veins after the fourth vaccination. By immunofluorescence staining analysis, pooled sera were examined for their specificity to αSyn in cells (HEK293/pAAV-αSyn) transfected with pAAV-αSyn plasmids (encoding human αSyn) and naive cells (HEK293) in the presence of αSyn110–130 or Spike-binder peptides at different concentrations (0, 0.7, 0.07 μg/μl). Cellular nuclei were labeled with DAPI (blue); Alexa488-conjugated polyclonal antibodies were applied to detect mouse IgG (green); scale bar = 20 μm.