AMPK phosphorylation and AC5 and AC6 gene expression in hearts from WT and AMPKα2-/- mice following TAC.

A, left ventricular mass/tibia length shows a similar hypertrophy in WT and KO mice; B, ejection fraction (%) showing the worsening of cardiac function in KO mice; C, representative western blots of αAMPK and ACC of three hearts per group performed on the same gel and mean values of phosphorylation evidencing an increase in αAMPK and ACC phosphorylation in WT-TAC mice only; D, following TAC, AC5 expression was significantly decreased in WT but not in KO mice while E, AC6 expression was decreased in both WT and KO mice. Values are means±SEM of 6–8 mice. *p$p$ $p$ $ $p<0.001 versus corresponding WT.