Functional assay of spätzle processing enzyme (<i>Tm-SPE</i>) for PLA₂ activity mediation.

(A) Mediation of sPLA2 activity induced by bacteria and rDSP1 via SPE. (B) Mediation of cPLA2 activity by bacteria and rDSP1 via SPE. L6 larvae were injected with E. mundtii (2.2 x 105 cells/larvae) or recombinant DSP1 (‘rDSP1’, 0.6 μg/larva). Naïve were injected with PBS. dsRNA specific to SPE or dsCON was injected 24 h before bacteria or rDSP1 injection. At 8 h after E. mundtii or rDSP1 injection, Fatbody and plasma was collected to determine cPLA2 and sPLA2 activity, respectively. dsRNA was made from a viral gene (CpBV302). Each treatment was replicated three times. Different letters above standard deviation bars denotes significant difference among means at Type I error = 0.05 (LSD test).