Evaluation of glucose consumption and lactate production in culture medium of HL-1 cells exposed to LG, NG, HG and IHG during 12 and 72h.

HL-1 cells were cultured at least during 3 weeks with normal glucose (5.5 mmol/l) then submitted to LG, NG, HG or IHG for 12 or 72h. Glucose (A and C) and lactate (B and D) concentrations were measured in the culture medium at different times (see Fig 1) to calculate glucose consumption and lactate production. Data are the means ± S.E.M of 4–6 independent experiments. Two-way ANOVA followed by the Tukey’s multiple comparisons test when evaluating the effect of glucose and time treatment. *, P $, P $ $, P $ $ $, P §, P §§§, P treatment (3, 48) = 23.85, Ptime (3, 48) = 7.03, P = 0.0005; FtreatmentXtime (9, 48) = 6.92, Ptreatment (3, 48) = 38.90, Ptime (3, 48) = 3.43, P = 0.0242; FtreatmentXtime (9, 48) = 1.63, P = 0.132. For 72h treatment and glucose consumption, Ftreatment (3, 79) = 49.66, Ptime (3, 79) = 3.602, P = 0.017; FtreatmentXtime (9, 79) = 12.26, Ptreatment (3, 79) = 22.69, Ptime (3, 79) = 4.308, P = 0.007; FtreatmentXtime (9, 79) = 2.819, P = 0.006. (PPTX)