Evaluation of medium lactate production in HL-1 cells culture with high and normal glucose.

HL-1 cells were cultured at least during 3 weeks either with normal (5.5 mmol/l) or high (25 mmol/l) glucose then submitted to 4 different regimens for 12h: LG, NG, HG or IHG. Lactate concentration was measured in the culture medium at 0, 2, 6, 10 and 12h to calculate lactate production. Data are the means ± S.E.M of 4 independent experiments. Two-way ANOVA followed by the Tukey’s multiple comparisons test when evaluating the effect of time treatment in HL-1 cells culture in normal or high glucose. For LG treatment, Ftreatment (1, 24) = 1.09, P = 0.3064; Ftime (3, 24) = 0.18, P = 0.9057; FtreatmentXtime (3, 24) = 0.43, P = 0.7270. For NG treatment, Ftreatment (1, 24) = 1.17, P = 0.2883; Ftime (3, 24) = 1.09, P = 0.3688; FtreatmentXtime (3, 24) = 0.03, P = 0.9914. For HG treatment, Ftreatment "F (1, 24) = 1.89, P = 0.1814; Ftime (3, 24) = 2.13, P = 0.1222; FtreatmentXtime (3, 24) = 0.09, P = 0.9605. For IHG treatment, Ftreatment (1, 24) = 0.05, P = 0.8237; Ftime (3, 24) = 1.47, P = 0.2467; FtreatmentXtime (3, 24) = 0.20, P = 0.8929. (PPTX)