MoMet2 represses <i>MoGLIK</i> expression via DNA methylation on HY, stress, and infection.

(A, B, C, D) Distribution of cytosine DNA methylation in three contexts in 350 bp of the MoGLIK promoter region in the Guy11, ΔMomet2 and the complemented mutant ΔMoMET2/MoMET2 HY. (B) HY added 1mM DTT (C) and punched rice (D) as measured by bisulfite sequencing. Sequencing data were analyzed using Kismeth software. CpG, lilac; CpHpG, blue; CpHpHp, green. At least 12 clones were sequenced per sample. (E) The relative expression of MoGLIK in different treatments. (F, G, H) Methylation levels of CpG, CpHpG, and CpHpHp in Guy11, ΔMomet2 and the complemented mutant ΔMoMET2/MoMET2. (I) Pathogenicity test on rice leaves. (J) Diseased leaf area analysis. Data are presented as a bar chart showing the percentage of lesion area analyzed by ImageJ. (K) Statistical analysis for each type of IH shape. Error bars represent the SD and different letters indicate significant differences (P < 0.05) tested by one-way ANOVA with Duncan’s post hoc test for (E and J).