Phytochemical characterization, antioxidant and antibacterial activity of Salvia officinalis (L.) extracts from the Tiaret region

This work aims the valorization of a medicinal plant known by its traditional use, Salvia officinalis L. (Lamiaceae), by phytochemical characterization and evaluation of the antioxidant and antibacterial activity of their extracts. The antioxidant activity was assessed by the DPPH method and the antibacterial potential was determined by the diffusion method. The quantitative determination revealed that the ethanolic extract has a content of 8.04% for polyphenolic content and 17.4 % for flavonoids. The DPPH radical scavenging activity of S. officinalis showed that the ethanolic extract of S. officinalis presented the higher antiradical effect manifested with IC50 of 0.106±0.001 mg/ml. In addition, the antibacterial activity showed the strong capacity of S. officinalis methanolic extract to inhibit B. subtilis, M. luteus, E. coli and S. aureus with a diameter inhibition zone of 27.06±1.49; 15.43±2.23; 11.6±0.52 and 11.5±2.17 mm respectively. While the activity of the ethanolic extract was 26.62±2.97 mm against B. subtilis, 16.51±2.36 mm against M. luteus, 13.62±0.55 mm for S. aureus, P. aeruginosa (12.30±1.59 mm). The macrodilution method (MIC) showed a range of 625 to >5000 µg/ml. The study of the antioxidant and antibacterial activity of extracts of S. officinalis suggested that this plant represented a natural source of bioactive molecules with very important biological activities