Fix for get VEP cache. Support Picard's new syntax for ReorderSam (REFERENCE -> SEQUENCE_DICTIONARY). Remove mitochondrial reads from ChIP/ATAC-seq calling. Add documentation describing ATAC-seq outputs. Add ENCODE library complexity metrics for ATAC/ChIP-seq to MultiQC report (see https://www.encodeproject.org/data-standards/terms/#library for a description of the metrics) Add STAR sample-specific 2-pass. This helps assign a moderate number of reads per genes. Thanks to @naumenko-sa for the intial implementation and push to get this going. Index transcriptomes only once for pseudo/quasi aligner tools. This fixes race conditions that can happen. Add --buildversion option, for tracking which version of a gene build was used. This is used dur...
Genome alignments for data generated in the project "Whole transcriptome analysis of thousands of FA...
BACKGROUND: The Affymetrix MitoChip v2.0 is an oligonucleotide tiling array for the resequencing of ...
Additional file 1. Criteria to evaluate ChIP-seq data quality. Illustrated calculation method of the...
1.2.4 (21 September 2020) Remove deprecated --genomicsdb-use-vcf-codec option as this is now the de...
1.2.5 (01 January 2021) Joint calling for RNA-seq variant calling requires setting jointcaller to b...
Set ENCODE library complexity flags properly for ChIP-seq. Thanks to @mistrm82. Fix greylisted peaks...
Update ChIP and ATAC bowtie2 runs to use --very-sensitive. Properly pad TSS BED file for ataqv TSS e...
Fix for bismark not being a supported aligner. Run ataqv (https://github.com/ParkerLab/ataqv) to cal...
Automated preprocessing of Next-Generation Sequencing data, including full (sc)ATAC-seq, ChIP-seq, a...
Update (v1.4.0-Chimeric): added example chimeric reads, one formed [presumably] during sample prep [...
This pipeline is designed for automated end-to-end quality control and processing of ATAC-seq and DN...
BACKGROUND: ATAC-seq (Assays for Transposase-Accessible Chromatin using sequencing) is a recently de...
Genome alignments for data generated in the project "Whole transcriptome analysis of thousands of FA...
BACKGROUND: The Affymetrix MitoChip v2.0 is an oligonucleotide tiling array for the resequencing of ...
Additional file 1. Criteria to evaluate ChIP-seq data quality. Illustrated calculation method of the...
1.2.4 (21 September 2020) Remove deprecated --genomicsdb-use-vcf-codec option as this is now the de...
1.2.5 (01 January 2021) Joint calling for RNA-seq variant calling requires setting jointcaller to b...
Set ENCODE library complexity flags properly for ChIP-seq. Thanks to @mistrm82. Fix greylisted peaks...
Update ChIP and ATAC bowtie2 runs to use --very-sensitive. Properly pad TSS BED file for ataqv TSS e...
Fix for bismark not being a supported aligner. Run ataqv (https://github.com/ParkerLab/ataqv) to cal...
Automated preprocessing of Next-Generation Sequencing data, including full (sc)ATAC-seq, ChIP-seq, a...
Update (v1.4.0-Chimeric): added example chimeric reads, one formed [presumably] during sample prep [...
This pipeline is designed for automated end-to-end quality control and processing of ATAC-seq and DN...
BACKGROUND: ATAC-seq (Assays for Transposase-Accessible Chromatin using sequencing) is a recently de...
Genome alignments for data generated in the project "Whole transcriptome analysis of thousands of FA...
BACKGROUND: The Affymetrix MitoChip v2.0 is an oligonucleotide tiling array for the resequencing of ...
Additional file 1. Criteria to evaluate ChIP-seq data quality. Illustrated calculation method of the...