HEPATOPROTECTIVE EFFECT OF COMBINATION OF TENDER LEAVES OF MANGIFERA INDICA LINN AND TENDER COCONUT WATER IN HEP G2 CELLS.

Liver is an important organ of metabolism and mortality due to liver diseases in India is 22.93%. Ayurveda claims the combination of paste of tender leaves of Mangifera indica Linn (M.indica) and tender coconut water is useful in liver disorders. But scarce scientific data is available on the protective effect of this combination in liver disorders. Hence the present study evaluated hepato-protective effect of this combination in HepG2 cells against carbon tetra chloride induced hepato-toxicity by using MTT assay. Aqueous and alcoholic extracts of the combination of drug was prepared as per the standard procedure. HepG2 cells were purchased from NCC, Pune. The experiments done with different concentrations of aqueous and alcoholic extract of the combination. Aqueous & alcoholic extract of tender leaves of M.indica and tender coconut water also tested separately. Silymarin was taken as positive control and untreated cells as negative control. All the experiments were done in triplicates and the percentage of cell viability was evaluated based on average of optical density readings. Data obtained were analysed statistically by ANOVA test. All the tested samples had hepatoprotective effect when compared to negative control and positive control at particular concentrations. The percentage of viability was more in combination especially at lower concentration of aqueous extract than that of silymarin. Thus the present study gathered scientific data on hepato protective activity of the combination of Tender leaves of M. indica and tender coconut water and thus traditional knowledge is validated