Sulfate-chloride sodium-magnesium mineral waters modulate neuroendocrine-immune complex and metabolism in healthy female rats

Popovych Daria V., Badiuk Nataliya S., Hrytsak Myroslava V., Ruzhylo Sofiya V., Mel’nyk Oksana I., Żukow Xawery. Sulfate-chloride sodium-magnesium mineral waters modulate neuroendocrine-immune complex and metabolism in healthy female rats. Journal of Education, Health and Sport. 2021;11(12):455-466. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2021.11.12.037 https://apcz.umk.pl/czasopisma/index.php/JEHS/article/view/JEHS.2021.11.12.037 https://zenodo.org/record/6320628 The journal has had 40 points in Ministry of Education and Science of Poland parametric evaluation. Annex to the announcement of the Minister of Education and Science of December 21, 2021. No. 32343. Has a Journal's Unique Identifier: 201159. Scientific disciplines assigned: Physical Culture Sciences (Field of Medical sciences and health sciences); Health Sciences (Field of Medical Sciences and Health Sciences). Punkty Ministerialne z 2019 - aktualny rok 40 punktów. Załącznik do komunikatu Ministra Edukacji i Nauki z dnia 21 grudnia 2021 r. Lp. 32343. Posiada Unikatowy Identyfikator Czasopisma: 201159. Przypisane dyscypliny naukowe:Nauki o kulturze fizycznej (Dziedzina nauk medycznych i nauk o zdrowiu); Nauki o zdrowiu (Dziedzina nauk medycznych i nauk o zdrowiu). © The Authors 2021; This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 02.12.2021. Revised: 20.12.2021. Accepted: 31.12.2021. SULFATE-CHLORIDE SODIUM-MAGNESIUM MINERAL WATERS MODULATE NEUROENDOCRINE-IMMUNE COMPLEX AND METABOLISM IN HEALTHY FEMALE RATS Daria V. Popovych1, Nataliya S. Badiuk1, Myroslava V. Hrytsak2,3, Sofiya V. Ruzhylo4, Oksana I. Mel’nyk5, Xawery Zukow6 1IY Horbachevs’kyi National Medical University, Ternopil’, Ukraine darakoz@yahoo.com 2SE Ukrainian Research Institute of Medicine of Transport, Odesa, Ukraine badiuk_ns@ukr.net 3Scientific group of Balneology of Hotel&Spa Complex "Karpaty", Truskavets’, Ukraine hrytsak.myroslava@gmail.com ira_barschyk@ukr.net 4Ivan Franko Pedagogical University, Drohobych, Ukraine 5Danylo Halyts’kyǐ National Medical University, L’viv, Ukraine omelnyk7@gmail.com 6Medical University of Bialystok, Bialystok, Poland xaweryzukow@gmail.com Background. Earlier in an experiment on rats, we showed that newly created sulfate-chloride sodium-magnesium drinking mineral waters of Truskavets’ spa has a significant modulating effect on the parameters of metabolism and the autonomic nervous, endocrine and immune systems. In this study, we combined data obtained on the same animals, in line with the concepts of neuroendocrine-immune complex and functional-metabolic continuum. Materials and Methods. Experiment was performed on 50 healthy female Wistar rats 230-290 g divided into 4 groups. Animals of the first group remained intact, using tap water from drinking ad libitum. Rats of the second (control) group for 6 days administered a single tap water through the tube at a dose of 1,5 mL/100 g of body mass. The rats of the main groups received the water "Myroslava" and "Khrystyna". The object of the study were the metabolic, neuro-endocrine and immune parameters. Results. The method of discriminant analysis revealed 31 parameters, according to which all four groups of animals differ from each other. Classification accuracy is 100%. Conclusion. The newly created sulfate-chloride sodium-magnesium drinking mineral waters of Truskavets’ spa has both similar and specific effects on the neuroendocrine-immune complex and metabolism at healthy old female rats with weekly use. This provides a basis for preclinical studies. Keywords: sulfate-chloride sodium-magnesium mineral waters, neuroendocrine-immune complex, metabolism, female rats