THE ROLE OF IN BOOSTING ANTIBIOTIC ACTION AGAINST IRRADIATED RESISTANT BACTERIA THROUGH NATURAL OUTER MEMBRANE PERMEABILIZERS

Objective: The objective of this research is to bring out novel approaches in order to revert pathogenic Gram-negative bacilli resistance by combining potent permeabilizers, conventional antibiotics and natural beta lactamase inhibitors increasing various antibiotics activity. Methods: This research was carried out at Services Hospital, Lahore from August 2018 to June 2019. Disk diffusion along with susceptibility assays was carried out for the antibiotic susceptibility in the presence phytochemicals tested concentrations of natural non-antibacterial. Gallic acid and Thymol were potentially facilitated and permeabilizers the antibiotics passage through outer membrane with the ability of release of LPS, Triton X-100 and sensitize bacteria to SDS. Results: Natural beta lactamase inhibitors and Permeabilizers combination with antibiotics initiated more resistant isolates susceptibility than antibiotic management through beta lactamase inhibitors alone. Distinct effects were also identified with 24.4 Gy in vitro gamma irradiation on beta lactamase activity, permeability barrier and outer membrane protein profiles of assessed isolates. Conclusions: Synergistic effects of researched antibiotics and natural phytochemicals leads to novel clinical varieties through outer membrane destabilization or beta lactamase enzyme inactivation; it enables the use of most cost-effective and older antibiotics against resistant strains. Keywords: Outer Membrane Permeability, Pathogenic, Beta Lactam Resistance, Gram-Negative Bacilli, Natural Beta Lactamase, Permeabilizers, Inhibitors and Gamma Irradiation