This dataset includes pupil size response (PSR), skin conductance response (SCR), electrocardiogram (ECG), electromyogram (EMG) and respiration measurements. Also included are CS and US information, keypress responses, keypress response times, key correctness and shock ratings for each of 21 healthy unmedicated participants (10 males and 11 females aged 22.9+/-3.0 years; discrepancies to published studies are due to misprints and exclusion of a subject with incomplete data, which was excluded in all conducted studies as well as here) participating in a classical (Pavlovian) discriminant delay fear conditioning task. Two pairs of CS+ and CS-, either complex or simple, were delivered with headphones (HD518, Sennheiser, Wedemark-Wennebostel, G...