Improvements to the Classical p-Nitrophenol Palmitate Spectrophotometrical Method.

Spectrophotometrical Lipase activity assay methods improvement and adaptation to semiautomatical worlflow. Organic solvents and pH influence on lipase activity determination.Proposed modifications to the classical p-NPP spectrophotometrical method for the determination of lipase activity of supernatants from lipolytic strains bring the opportunity to process more samples under different conditions, saving experimentation time and reducing the amount of reagents needed for every sample