IN VITRO STUDIES ON PRELIMINARY PHYTOCHEMICAL SCREENING OF PLEUROTUS FLORIDA CULTIVATED ON PADDY STRAW

Mushrooms are white rot fungi regarded as one of the well known food and possessing various kinds of biopharmaceuticals compounds. Mushrooms are superior to many vegetables based on their nutritional value and it contains 40 - 49% of proteins. The present study consolidates in the aspects of cultivation of Pleurotus florida mushroom with phytochemical analysis. Pleurotus florida mushroom was cultivated by using paddy straw. Oyster mushroom accounted for 14.2% of the total world production of edible mushroom.P. florida was successfully grown on PDA. The fresh fruit bodies of mushroom were harvested, dried, powdered and extracted (by using ethanol) using soxhlate apparatus. The phytochemical characters of Pleurotus florida of water and ethanol extract investigated.The aqueous extract of Pleurotus florida was found to contain alkaloids, flavonoids, terpenoids, steroids and cardiac glycosides. Whereas ethanol extract of Pleurotus florida showed, the presence of alkaloids, flavonoids, terpenoids, saponins and steroids. Aqueous, ethanolic and methanolic extracts contains high amount of phenolic compounds, glycosides and flavonoids based on the phytochemical screening. CONCLUSION: In the present investigation was carried out on cultivation of Pleurotus florida and screening of preliminary phytochemicals. Pleurotus florida was cultivated on sterilized paddy straw. Environmental factors of pH (6.5) and temperature (25– 300C) were maintained between mycelia growth to attain harvesting stage. Cultivation of the growth was observed in raw materials in 18-25 days and first harvest can be made on 19th day itself it implies that the growth of the mushroom. Total yield was one kg for one bed. Preliminary phytochemical compounds were screened of Pleurotus florida with different extract of aqueous and ethanol solvent. Alkaloids, flavonoids, terpenoids, steroids and cardiac glycosides were presence in aqueous extract of Pleurotus florida. Whereas ethanol extract of Pleurotus florida was contain alkaloids, flavonoids, terpenoids, saponins and steroids. Some phytochemical ingredients Phenols, tannins, pholobatannins and anthroquinones were absence in both extracts. Further study can be aimed at to identify the bioactive compounds and determine the cultivation of Pleurotus florida on paddy Straw