Neuroendocrine-immune complex as the mirror of the state of exchange of nitrogenous metabolites at rats

Popovych Igor L., Kuchma Igor L., Ruzhylo Sofiya V., Zukow Walery, Gozhenko Anatoliy I. Neuroendocrine-immune complex as the mirror of the state of exchange of nitrogenous metabolites at rats. Journal of Education, Health and Sport. 2021;11(8):55-65. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2021.11.08.006 https://apcz.umk.pl/czasopisma/index.php/JEHS/article/view/JEHS.2021.11.08.006 https://zenodo.org/record/5195578 The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019. © The Authors 2021; This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 29.07.2021. Revised: 30.07.2021. Accepted: 13.08.2021. NEUROENDOCRINE-IMMUNE COMPLEX AS THE MIRROR OF THE STATE OF EXCHANGE OF NITROGENOUS METABOLITES AT RATS Igor L. Popovych1,4, Igor L. Kuchma1, Sofiya V. Ruzhylo2, Walery Zukow3, Anatoliy I. Gozhenko1 1Ukrainian Scientific Research Institute for Medicine of Transport, Odesa, Ukraine igorkuchma@ukr.net; prof.gozhenko@gmail.com 2Ivan Franko Pedagogical University, Drohobych, Ukraine doctor-0701@ukr.net 3Nicolaus Copernicus University, Torun, Poland w.zukow@wp.pl 4Bohomolets’ OO Institute of Physiology of National Academy of Sciences, Kyїv, Ukraine i.popovych@biph.kiev.ua Abstract Background. Earlier we found that even in intact rats, certain parameters of nitrogenous metabolism fluctuate in a fairly wide range, which further expands in cases of prolonged water loads. Based on this, we have been created three groups that are homogeneous in the parameters of nitrogenous metabolism. We have been shown that each cluster is accompanied by a specific constellation of immune parameters. In this study, an attempt will be made to supplement the immune accompaniment of each constellation of nitrogenous metabolites with parameters of the autonomic nervous and endocrine systems. Material and methods. Experiment was performed on 60 healthy female Wistar rats, both intact and loaded with different mineral waters. Immune status was assessed by thymocytogram, splenocytogram, blood leukocytogram and immunocytogram, as well as by phagocytosis parameters of blood neutrophils and monocytes. The state of autonomous regulation assessed by HRV. The plasma levels of the hormones of adaptation: corticosterone, triiodothyronine and testosterone (by the ELISA) were determined as well as mineralocorticoid, calcitonin and parathyroid activity calculated by their electrolyte markers. Results. 9 neuro-endocrine and 17 immune parameters were identified, the set of which three clusters of nitrogen metabolism clearly differ from each other. The overall accuracy of the classification is 93,3%. Conclusion. The variety of states of exchange of nitrogenous metabolites is accompanied by specific constellations of 26 parameters of neuro-endocrine-immune complex. Key words: bilirubin, creatinine, urea, uric acid, immunity, HRV, hormones, rats