Bio-activity guided fractions, isolation and antimicrobial evaluation of active constituents of Plumbago zeylanica Linn.

ABSTRACT Plumbago zeylanica belonging to the family plumbaginaceae , proved to be a richer source of components with possible pharmacological values. Isolated from the root of this plant crude extracts were exhibited antimicrobial, antidermatophyte property, the active extracts were subjected for isolation of active constituent by using different techniques. On the basis of antimicrobial activity P. zeylanica have been chosen for the present study. The Petroleum ether, chloroform, ethanol and distilled water extracts of roots of P. zeylanica were prepared and these crude extracts were screened for their antimicrobial activity by agar dilution method. All the crude extracts has shown promising antimicrobial activity and the results were recorded in minimum inhibition concentration (MIC) values. The crude cold methanol extract showed inhibition at a concentration of 35 mg/ml for S. aureus. 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