Environmental fluctuations accelerate molecular evolution of thermal tolerance in a marine diatom

Data (other than molecular data, which are in the Sequence Read Archive as Bioproject PRJNA397360) for "Environmental fluctuations accelerate molecular evolution of thermal tolerance in a marine diatom" https://doi.org/10.1038/s41467-018-03906-5. 1. "Data Figure01 natcomms Schaum et al.xlsx" contains data to analyse (and produce Figure 1A and B) for the fitness trajectories and thermal tolerance curves of growth. 2. The raw growth rate data for the thermal tolerance curves of growth are provided in separate files: Data_thermal_tolerance_growth_t000.csv,Data_thermal_tolerance_growth_t100.csv,Data_thermal_tolerance_growth_t300.csv contain the growth rates across a temperature gradient for the ancestor (t000), the evolved samples after 100 generations (t100) and after 300 generations (t300). In these files, assayT is the assay temperature in degrees C, selected is the selection regime, and bore is the biological replicate. r is the growth rate, K is carrying capacity. quasi r2 (or 2) is a measure of the goodness of the fit that the growth rate was calculated from. pa is an identification column that gives a unique name to individual bioreplicate/ assay temperature/selection regime combinations. 3. Photosynthesis and respiration data for Figure 2 a-e is contained in the files "tdep_metab_t300_32" for 32 degree C data after 300 generations "tdep_metab_t000" for the ancestor "tdep_metab_all_else" for all other metabolism data, i.e. photosynthesis and respiration data after 100 and 300 generations of evolution in all selection environments. As this is a large amount of data, they are saved in RDS format. they can be opened in any version of R using the command readRDS(name of file here). 4. File "Data_cell_volume_CNratio_etc" contains pg C per cell, CN ratios, NP ratios, and CN ratios, as well as cell volume and chlorophyll to carbon ratios for all selection regimes in the ancestor and the evolved lineages. 5. File "Data_cell_size_RNAProtein_etc.csv" contains data on size in µm, RNA and protein per cell, as well as silicate per cell