Method of Simultaneous Estimation of Nitazoxanide and Ofloxacin from Bulk and Tablet Dosage Form Using RP-HPLC

For the simultaneous determination of Nitazoxanide and Ofloxacin in tablet dosage form, a simple reverse phase liquid chromatographic method was developed and validated. A mobile phase containing acetonitrile, methanol, and 0.4 M citric acid (60:30:10, v/v/v) was used for the separation. Thermo C18 (4.6mm*250 mm, 5 ) column was used, with a flow rate of 1 mL/min and UV detection at 300 nm. Nitazoxanide and ofloxacin had retention times of 3.071 and 12.47 minutes, respectively. The described method was linear over a concentration range of 5-30 g/mL for Nitazoxanide (r2>0.999) and 2-12 g/mL for Ofloxacin (r2>0.998). The mean percentage recovery for Nitazoxanide was 99.96% and 101.05% for Ofloxacin. Nitazoxanide and Ofloxacin had limits of detection (LOD) of 0.3788 and 0.0929 g/mL, respectively. The limit of quantification (LOQ) for Nitazoxanide and Ofloxacin, respectively, was 1.1479 g/mL and 0.2816 g/mL. The study's findings revealed that the proposed RP-HPLC method is simple, fast, precise, accurate, and cost effective, making it suitable for routine determination of Nitazoxanide and Ofloxacin in bulk and tablet dosage forms