A COMPREHENSIVE PROTOCOL OF GENE SEQUENCING FOR BACTERIAL DIVERSITY FOLLOWING ILLUMINA GENE SEQUENCING PROTOCOL: A METAGENOMIC APPROACH

Microorganisms are key components of the soil biota. Earthworms are an essential part of the soil fauna in most global soils, represent a significant proportion of the soil biomass and are regarded as a useful indicator of soil health and quality. They maintain the dynamic equilibrium and regulate soil fertility. Very few studies have been done in bacterial population. The purpose of this work is undertaken to study the comprehensive protocol for Illumina Gene Sequencing for bacterial diversity count followed by extraction of DNA from earthworm gut. Two earthworm species were used in this work, Pheretima posthuma and Eisenia fetida which were named as 1A and 2A respectively. The DNA of the two earthworms gut samples were extracted following c-TAB and Phenol: Chloroform Extraction method. The quantity and quality of the extracted DNA was high and the bacterial count of the extracted DNA gut samples following Illumina Gene sequencing protocol is very productive. This method can be used for other species for bacterial study