ІNFLUENCE OF THE DRUG THIOTRIAZOLINE ON IMPAIRED INDICATORS OF FREE RADICAL OXIDATION AND ANTIOXIDANT PROTECTION IN THE LUNGS UNDER CONDITIONS OF DEVELOPMENT OF CONTACT DERMATITIS AND EXPERIMENTAL PNEUMONIA

Regeda-Furdychko M. M. ІNFLUENCE OF THE DRUG THIOTRIAZOLINE ON IMPAIRED INDICATORS OF FREE RADICAL OXIDATION AND ANTIOXIDANT PROTECTION IN THE LUNGS UNDER CONDITIONS OF DEVELOPMENT OF CONTACT DERMATITIS AND EXPERIMENTAL PNEUMONIA. Актуальні проблеми транспортної медицини / Actual problems of transport medicine / 2020;1(59):154-160. ISSN 1818-9385 DOI http://dx.doi.org/10.5281/zenodo.3744414 http://aptm.org.ua ІNFLUENCE OF THE DRUG THIOTRIAZOLINE ON IMPAIRED INDICATORS OF FREE RADICAL OXIDATION AND ANTIOXIDANT PROTECTION IN THE LUNGS UNDER CONDITIONS OF DEVELOPMENT OF CONTACT DERMATITIS AND EXPERIMENTAL PNEUMONIA M. M. Regeda-Furdychko Lviv Medical Institute Summary The purpose of this study was to investigate the features of changes in the processes of lipoperoxidation and antioxidant protection in the lungs with the development of experimental contact dermatitis (EСD) and experimental pneumonia (EP) before and after administration of the antioxidant thiotriazoline. Materials and methods. Researches were conducted on guinea pigs, divided into five groups: I — control, II — 4th day of expirement, III — 8th day of contact dermatitis development and experimental pneumonia, IV — 10th day and V — 18 th day of model processes and VI group — guinea pigs with ECD and EP after treatment with thiotriazoline during 10 days (from 8 th to the 18 th days of the experiment). Early period included groups of animals on the 4th and 8th days of experiment. The late one — guinea pigs on the 10th and 18th days of ECD and EP. Experimental contact dermatitis was simulated by method of Volkovoj V.A. (2010). EP was called by the method of Shlyapnikov V.N., Solodov T.L. (1998). Thiotriazoline was administered intramuscularly at a dose of 100 mg per 1 kg of weight daily from the 8 th to the 18 th days of the experiment. Condition of free radical lipid oxidization in the lungs was determined on maintenance content of malonic dialdehyde by method of Corobeynikov E.G. (1989) and diene conjugates by method of Gavrylov V.G., Myshkorudna M. I. (1989). The degree of activity of antioxidant defence was estimated on maintenance enzymes — superoxidedismutase by method of R. Fried (1975), catalase by the method of B. Holmes, C. Masters (1970), glutationperoxidase method of Arkhipova O. G. (1988) and glutationreductase method of Moina V.M. (1986). Results and discussion. In the experiment on guinea pigs it is shown that the experimental contact dermatitis and experimental pneumonia is accompanied by impaired functional status of the prooxidant and antioxidant systems. The study found the increase of diene conjugates and malonic dialdehyde, decreased activity of superoxidedismutase, catalase, glutathionereductase and glutathione peroxidase in guineapig lungs, especially pronounced on the 18th day of combined experimental pathology. The antioxidant corrective action of thiotriazoline on the indicated indicators at ECD and EP was detected. Key words: contact dermatitis, pneumonia, lipid peroxidation, antioxidant system, thiotriazoline