Impedance-based phenotypic for SLC1A3 using HEK JumpIn SLC1A3 OE cells

Human SLC1A3 (solute carrier family 1 member 3) or EAAT1 (excitatory amino acid transporter 1), is a high affinity sodium-, proton-, and potassium-dependent glutamate transporter located in the plasma membrane.The phenotypic impedance-based assay to measure excitatory amino acid transporter (EAAT1, SLC1A3) enables detection of EAAT1 activity by uptake-induced changes of cell morphology.The mechanism behind the glutamate-induced response in dox-treated JumpIn-EAAT1 cells was attributed to initial cell swelling and subsequent spreading