High-resolution spatial multi-omics datasets

Supplementary raw data. The raw microscopy data are not uploaded owing to their large size (6.8 Tb), but are available upon reasonable request (Long Cai: lcai@caltech.edu, Yodai Takei: ytakei@caltech.edu). These supplementary data contain additional files for RNA seqFISH+, DNA seqFISH+, and sequential immunofluorescence from cell culture and adult mouse cerebellum experiments. DNA seqFISH+ datasets (provided as a tar.gz folder for each replicate): Super-resolved DNA spot locations by DNA seqFISH+ along with sequential immunofluorescence intensity at the rounded voxel location. RNA seqFISH datasets (provided as a zip folder): Super-resolved mRNA or intron spot locations. Sequential immunofluorescence table (provided as a csv file): Mean voxel intensity of each immunofluorescence marker per nucleus for the adult mouse cerebellum datasets. Note that voxel sizes are 103 nm for x and y, and 250 nm for z in our experimental setting. Please find the uploaded readme.txt file for more details