EVALUATION OF THE MICROBIOLOGICAL QUALIFICATION OF BLOOD DONATION AT THE AVICENNE MILITARY HOSPITAL IN MARRAKECH

Introduction: The microbiological qualification of blood donations has made great progress in terms of infectious risk. The use of new technologies and methods in transfusion has been an element of reflection as a new approach to search for viruses in blood donations, which has contributed to the improvement of the quality of screening and transfusion safety. The objective of our work was to evaluate the microbiological qualification techniques of blood donation at the Blood Transfusion Center (BTC) of the Avicenne Military Hospital of Marrakech (AMH) in comparison with what is done nationally and internationally. Materiels and Methods: We conducted a retrospective descriptive, comparative and evaluative study of 8221 blood donations collected at the BTC (old and new) of the AMH of Marrakesh, carried out over a five-year period from January 1, 2016 to December 31, 2020. Results: The prevalence of serological markers of hepatitis B is 0.28% that of hepatitis C is 0.17%, that of HIV is 0.09%, and that of syphilis is 0.40%. The screening of hepatitis B, C and HIV viruses at the BTC of the Avicenne Military Hospital of Marrakech, is carried out by the ELISA technique (enzyme-linked immunosorbent assay) in sandwich and combined of 4th generation, automated on EVOLIS "Twin plus".Syphilis screening is based on the hemagglutination technique by the TPHA test. The control of positive and doubtful cases is carried out using the chemiluminescence technique (CLIA) in the virology department. Conclusion: The acquisition of new screening techniques in our laboratory has become a necessity due to the development of new screening methods in recent years that are more sensitive and specific, such as the introduction of viral genomic screening (VGS) into the routine of viral screening tests