The effect of aeromoniasis on protein and DNA content and histopathology of muscle and gill in adaptively immunized fish

ABSTRACT The present investigations were designed to estimate the content of protein and DNA and to understand the histopathology in muscle and gill of fish infected with repeated doses of A. liquefaciens. Two varied multiple doses of pathogen, 10-2 + 10-2 CFU/Fish and 10-3 + 10-3 CFU / Fish were injected intramuscularly to two different groups of fish A and B (66 in each group) respectively; two groups a and b were served as controls for comparison. Muscle and gill were selected for studying the content of protein and DNA and for histopatholgoical changes. Six fish from the experimental and control groups were necropsied at hour 1, 3,6,12,18,24,36,48,72,96 and 216 of experimental period. Tissues of muscle and gill were separated and analyzed for total protein and DNA. Estimation of protein and DNA content and histopathological findings in both the experimental and control groups of fish were done following standard methods. Both the infected groups of fish showed clinical symptoms such as weakness, anorexia, scale falling, hemorrhages, skin ulcers and necrosis of viral organ. A. liquefaciens, one of the most important pathogens of the out breaks in fresh water fish effected the level of protein and DNA in muscle and gills from hour 1 to 216 of experimental period. The stress caused by A.liquefacaensis contributing to the disturbed physiology in fish. Fish which were exposed to multiple infection of A. liquefacaens did not show resistance and in these two groups of fish A (10-2 + 10-2 CFU/Fish) and B (10-3 + 10-3 CFU/Fish) there is a marked decrease in the level of protein and DNA in muscle and gill from hour 1 to 216 with few exceptional following the intramuscular injection, lesions developed in the visceral organs such as kidney, liver and spleen. 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