AN OVERVIEW OF CAPILLARY ELECTROPHORESIS

Capillary electrophoresis is an effective separation technique where the ions are separated based on their electrophoretic mobility under an applied voltage. Capillary electrophoresis is most predominately used because it gives faster results and provides a high-resolution separation. It is one of the useful techniques as there is a large range of detection methods available. CE is an alternative for traditional methods such as gel electrophoresis and liquid chromatography and is employed to detect both high and low affinity molecular interactions, and separation of both charged and non-charged molecules. CE classified according to mode of separation on the basis of differences in charge, size and frictional force, offers fast separations with excellent efficiency. CE is an effective analytical tool for assay of pharmaceutical API including determination of drug related impurities. It possess other versatile applications like chiral, and bioanalysis of pharmaceutical API This review focuses on various aspects of capillary electrophoresis and CE-based separation modes with some advantages and disadvantages along with applications. KEYWORDS: Capillary Electrophoresis, High Resolution, Frictional Force, liquid chromatograph