BIOMOLECULAR IDENTIFICATION OF STAPHYLOCOCCUS AUREUS AND SCREENING OF METHICILLIN RESISTANT STRAIN FROM PUS /WOUND SAMPLE IN TERTIARY CARE HOSPITAL QUETTA

The aim of this study was the bio-molecular identification of Staphylococcus aureus and screening of methicillin resistant strain. To evaluate the study, 60 samples of pus and wound were collected from Bolan Medical Complex Hospital Quetta, Balochistan during period from September 2016 to December 2017. Out of 60 samples processed for isolation, ten (10) were found positive for staphylococcus aureus. Isolates were recognized by growth characteristic on selective media, Gram staining and different biochemical tests like oxidase, catalase, coagulase, motility, thermonuclease (DNase), haemolysis and mannitol fermentation and citrate utilization test. All the isolates found positive through biochemical tests also confirmed by PCR based on 16S rRNA and Nuc gene. In the present study antibiotic sensitivity and resistance pattern of Staphylococcus aureus was recorded to the following antibiotics; Tetracycline, Ciprofloxacin, Ceftriaxone, Vancomycin, Penicillin, Ampicillin, gentamicin, Cloxacillin, Methicillin and Novobiocin were 30%, 80%, 70%, 100%, 10%, 10%, 70%, 40%, 80% and 30% respectively. Two isolates which showed resistant to methicillin were also found resistant to all other antibiotics except Vancomycin. Keyword: MRSA, Staphylococcus aureus, antibiotic susceptibility, Biomolecular identification