FIG. 23 in Fungal Biodiversity Profiles 81-90

FIG. 23. — Maximum parsimony Strict consensus tree illustrating the phylogeny of Serpula dendrocalami C.L. Zhao, sp. nov., and related species in Serpula based on nLSU sequences. Branches are labeled with parsimony bootstrap values (before slash) higher than 50% and Bayesian posterior probabilities (after slash) equal to and more than 0.95. The nLSU dataset included sequences from 16 fungal specimens representing 8 species. The dataset had an aligned length of 1411 characters, of which 1158 characters are constant, 69 are variable and parsimony-uninformative, and 184 are parsimony-informative. Maximum parsimony analysis yielded 4 equally parsimonious trees (TL = 321, CI = 0.854, HI = 0.146, RI = 0.947, RC = 0.808). Best model for the nLSU dataset estimated and applied in the Bayesian analysis: GTR+I+G, lset nst = 6, rates = invgamma; prset statefreqpr = dirichlet (1,1,1,1). Bayesian analysis and ML analysis resulted in a similar topology as MP analysis, with an average standard deviation of split frequencies = 0.003841 (BI). This phylogenetic analysis demonstrated that our species was closely related to S. similis (Berk. & Broome) Ginns. Nuclear LSU region was amplified with primer pairs LR0R and LR7 (http://www.biology.duke.edu/fungi/mycolab/primers.htm). The PCR procedure for nLSU was as follows: initial denaturation at 94 °C for 1 min, followed by 35 cycles at 94 °C for 30 s, 48 °C 1 min and 72 °C for 1.5 min, and a final extension of 72 °C for 10 min. The PCR products were purified and directly sequenced at Kunming Tsingke Biological Technology Limited Company. All newly generated sequences were deposited at GenBank (Table 1). Branches are labeled with parsimony bootstrap values (before slash) higher than 50% and Bayesian posterior probabilities (after slash) equal to and more than 0.95.Published as part of Wang, Xiang-Hua, Das, Kanad, Bera, Ishika, Chen, Yu-Hui, Bhatt, Rajendra Prasad, Ghosh, Aniket, Hembrom, Manoj Emanuel, Hofstetter, Valérie, Parihar, Arvind, Vizzini, Alfredo, Xu, Tai-Min & Zhao, Chang-Lin, 2019, Fungal Biodiversity Profiles 81-90, pp. 57-95 in Cryptogamie, Mycologie 20 (5) on page 90, DOI: 10.5252/cryptogamie-mycologie2019v40a5, http://zenodo.org/record/781489