This dataset includes skin conductance response (SCR), electrocardiogram (ECG) and respiration measurements. Also included are CS and US information, keypress responses and keypress response times for 20 healthy unmedicated participants (7 males and 13 females aged 26.15+/-4.15 years) participating in a classical (Pavlovian) discriminant delay fear conditioning task. CS were a red and a blue rectangle. US consisted of 0.5 s square electric pulses with 0.2 ms duration and 10 Hz frequency. SOA between the CS onset and US was 3.5 s. CS and US co-terminated. The ITI was randomly determined on each trial to be 7, 9, or 11 s.See the readme file for more detail. Data are stored as .mat files for use with MATLAB (The MathWorks Inc., Natick, USA) i...