Fig. 6 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS/MS data of reference desulfoglucosinolates

Fig. 6. Lack of the aliphatic glucosinolate (GSL) Pren (107) in B. vulgaris and spiking of pure (intact) Pren for establishing the limit of detection. A. Total ion chromatogram for the three dominating peaks in G-type B. vulgaris (dGSL preparation). B. Extracted ion trace for desulfo Pren in the same extract as A, showing lack of detection. C, D, E. Results of serial spiking of the crude extract with serial 10-fold dilutions of Pren before the desulfation procedure, showing linearity also at low levels and ability to detect trace levels.Published as part of Agerbirk, Niels, Hansen, Cecilie Cetti, Olsen, Carl Erik, Kiefer, Christiane, Hauser, Thure P., Christensen, Stina, Jensen, Karen R., Ørgaard, Marian, Pattison, David I., Lange, Conny Bruun Asmussen, Cipollini, Don & Koch, Marcus A., 2021, Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS/MS data of reference desulfoglucosinolates, pp. 1-19 in Phytochemistry (112658) 185 on page 12, DOI: 10.1016/j.phytochem.2021.112658, http://zenodo.org/record/825967