Temporal dynamics of soil fungi in a pyrodiverse dry-sclerophyll forest

Fire is a major evolutionary and ecological driver that shapes biodiversity in forests. While above-ground community responses to fire have been well-documented, those below-ground are much less understood. However, below-ground communities, including fungi, play key roles in forests and facilitate the recovery of other organisms after fire. Here, we used internal transcribed spacer (ITS) meta-barcoding data from forests with three different times since fire [short (3 years), medium (13–19 years) and long (>26 years)] to characterize the temporal responses of soil fungal communities across functional groups, ectomycorrhizal exploration strategies and inter-guild associations. Our findings indicate that fire effects on fungal communities are strongest in the short to medium term, with clear distinctions between communities in forests with a short time (3 years) since fire, a medium time (13–19 years) and a long time (>26 years) since fire. Ectomycorrhizal fungi were disproportionately impacted by fire relative to saprotrophs, but the direction of the response varied depending on morphological structures and exploration strategies. For instance, short-distance ectomycorrhizal fungi increased with recent fire, while medium-distance (fringe) ectomycorrhizal fungi decreased. Further, we detected strong, negative inter-guild associations between ectomycorrhizal and saprotrophic fungi but only at medium and long times since fire. Given the functional significance of fungi, the temporal changes in fungal composition, inter-guild associations and functional groups after fire demonstrated in our study may have functional implications that require adaptive management to curtail.ExcelFunding provided by: ARC Linkage Project (Australian Research Council, Parks Australia and the Australian Department of Defence)*Crossref Funder Registry ID: Award Number: LP170100152Please see the README document ("README_dryad.csv") and the accompanying published article: Bowd, E. J., Egidi, E., Lindenmayer, D. B., Wardle, D. A., Kardol, P., & Foster, C. (2023). Temporal dynamics of soil fungi in a pyrodiverse dry-sclerophyll forest. Molecular Ecology, 32(15), 4181–4198. https://doi.org/10.1111/mec.1703