Fig. 1 in Alterations in nepetalactone metabolism during polyethylene glycol (PEG)- induced dehydration stress in two Nepeta species

Fig. 1. The exposure of N. rtanjensis and N. argolica ssp. argolica shoots to PEG-induced dehydration stress under in vitro conditions resulted in the alterations of morphological and physiological parameters. As revealed by SEM of leaf surfaces of non-treated (a and c) and PEG-treated (b and d) N. rtanjensis and N. argolica ssp. argolica plants, peltate glandular trichomes (PGT) preserve their morphology, while stomata (white arrows) are closed in PEG-treated leaves at 6 days of PEG treatment (6DPT). Morphological differences between non-treated (e and g) and PEG-treated plants (f and h) at 6DPT indicated tissue dehydration and turgor loss in both model species. As a measure of dehydration stress, fresh weight (FW) of shoots (i and k) and their relative water content (RWC) (j and l) were traced throughout 6 days of PEG treatment in both N. rtanjensis and N. argolica ssp. argolica shoots. PEG-treated N. rtanjensis (yellow bars) and N. argolica ssp. argolica plants (green bars) are compared with their respective controls (grey bars) at 1DPT, 3DPT and 6DPT. Error bars represent SE of three biological replicates. Asterisks (*) denote significant differences between PEG-treated and non-treated plants (unpaired t-test, *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001). Abbreviations: DPT-days of PEG treatment. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)Published as part of Aničić, Neda, Matekalo, Dragana, Skorić, Marijana, Živković, Jasmina Nestorović, Petrović, Luka, Dragićević, Milan, Dmitrović, Slavica & Mišić, Danijela, 2020, Alterations in nepetalactone metabolism during polyethylene glycol (PEG)- induced dehydration stress in two Nepeta species, pp. 1-12 in Phytochemistry (112340) 174 on page 3, DOI: 10.1016/j.phytochem.2020.112340, http://zenodo.org/record/829477