In Silico docking studies on thrombolytic properties of Homoeopathic Medicine Crataegus oxycantha by activation of tissue-Plasminogen Activator in the treatment of Vascular Thrombosis

ABSTRACT: The main aim of this study is to analyse the effective thrombolytic activity of Homoeopathic Medicine Crataegus oxyacantha in the treatment of Vascular Thrombosis. A thrombus may form if a vein or an artery or surrounding tissue is damaged. The thrombi can affect the flow of blood through arteries or veins and if the thrombi gets detached from the endothelium of the blood vessel, it lodges in vital organs such as the brain, heart, and lungs, which pose a serious threat (Lyaker et al., 2013)[13]. Plasminogen activation is a key event in the fibrinolytic system that results in blood clot dissolution and promotes cell migration and tissue remodelling. In this study, a total of 4 phytochemical constituents from Crataegus oxycantha were selected for docking studies. Crataegolic acid, Epicatechin, and Vitexin showed significant binding against the target plasminogen. The present work was carried out on computer-aided molecular modelling and the strength of the ligand was validated using binding energy. The bioactive compounds like Epicatechin and Vitexin exhibited good dock scores and these phytochemical constituents of Crataegus oxycantha may exert promising thrombolytic activity. Further in vivo study and RCT are required to validate the thrombolytic activity of Homoeopathic Medicine Crataegus oxycantha