Pixelwise H-score: a novel digital image analysis based-metric to quantify membrane biomarker expression from immunohistochemistry images

The dataset consists of whole slide image scans of immunohistochemistry images of human tumor resections. Specifically, there are 3 different sets of images pertaining to biomarkers PD-L1 (n = 24), 5T4 (n = 21) and P-cadherin (n = 30). The IHC images for PD-L1 and P-cadherin are in svs file format, while the IHC images for 5T4 are in NDPI file format. All the images can be opened using the following software packages: 1. Aperio ImageScope software; free image viewer, https://www.leicabiosystems.com/digital-pathology/manage/aperio-imagescope/ 2. QuPath software; free viewer and image analysis software, https://qupath.github.io/ 3. HALO software; requires a license, http://www.indicalab.com 4. Visiopharm software; requires a license, http://www.visiopharm.co