STABILITY INDICATING RP-HPLC METHOD FOR ESTIMATION OF THIOCOLCHICOSIDE IN PARENTERAL DOSAGE FORMS

ABSTRACT A rapid, simple, precise stability indicating RP-HPLC method for the determination of thiocolchicoside on C18 column (250mm × 4.0mm, 5µm) have been developed. A mobile phase consisting of ammonium acetate: methanol (50:50) was used. The flow rate was 0.8ml/min by UV detection at 256 nm. The specificity of the method was ascertained by forced degradation studies by alkali, acid degradation and oxidation, photolysis. Beer law was obeyed over the range of 1-10µg/ml, correlation co efficient as 0.9996. This method was also successfully applied for routine analysis of thiocolchicoside in parenteral dosage forms. KEYWORDS: RP-HPLC, Thiocolchicoside, Forced degradation, Stability, Parenteral dosage form