ANTIBACTERIAL, ANTIFUNGAL, PHYTOTOXIC AND BRINE SHRIMP LETHALITY BIOASSAY OF ACACIA JACQUEMNOTII AND RHAMNUS PERSICA

The aim of this study was to investigate antibacterial, antifungal, phytotoxic and Brine shrimp lethality bioassay of Acacia jacquemnotii and Rhamnus persica. Dichloromethane and methanol extracts of the plants were evaluated for described activities. Agar tube diffusion method was employed for study of antibacterial and antifungal potential. Methanol extract of Acacia jacquemontii exhibited moderate antibacterial activity against some of the tested microorganisms. Non significant antifungal activity was shown by both plant extracts. Phytotoxic potential of both plant extracts was analyzed by using Lemna minor phytotoxic bioassay. The methanol extract of root bark of Acacia jacquemontii showed 35% growth inhibition of Lemna minor where as both dichloromethane and methanol extract of aerial parts of Rhamnus persica showed 40 % growth inhibition of Lemna minor at higher concentration. In Brine shrimp Lethality Bioassay, The dichloromethane extract of aerial parts of Rhamnus persica exhibited 56.66 % mortality. Similarly methanol extract of (root bark) of Acacia jacquemontii and aerial parts of Rhamnus persica showed 53.33 and 66.66 mortality respectively at dose of 1000µg/ml. These results confirm that both plant extracts possess significant phytotoxic as well as cytotoxic potential, where as moderate antibacterial effect was also observed, thereby providing worthy justification for isolation of novel bioactive compounds. Further study is needed to screen out cytotoxic potential of both plants against various cell lines. Keywords: Acacia jacquemontii, Rhamnus persica, Antibacterial, Antifungal, Phytotoxic, Cytotoxi