Figure S1. Western blot analysis of effectors in cell-associated and supernatant proteins of ORS3257 WT and ∆T3SS strains. (a) Western blots were performed with an anti-His6 antibody. The gene accession number corresponding to the tagged protein for each strain is indicated on the right and the size of the band on the left. (b) Western blot with anti-GFP antibody of cells and supernatant (sup) proteins of ORS3257 WT and strains producing His6 tagged proteins. The gene number corresponding to the tagged protein for each strain is indicated
<p>The strain used for co-IP expressed the respective proteins in suitable amounts (cell extracts); ...
<p>Proteins released in supernatant were revealed with non position-specific antiHis primary antibod...
<p><b>(A)</b> Western blot of acid-solubilized cell lysates (prepared from bacterial cells grown in ...
(A) The detection of C11orf83_6xH constructs by western blotting. McCoy cells were infected with the...
<p>Lines 1–2 show induced Hsp70 class protein, lines 3–4 constitutive Hsp70 and line 5 a 70 kD size....
<p>Spore coat proteins were extracted and analyzed by western blotting with anti-influenza M2 mAb. N...
<p>(A) COS-1 cells transfected with pZeoSV2 empty vector and labeled with <sup>35</sup>S-methionine....
<p><u>Panel A</u>. Western blot analysis of whole cell lysates of in vitro cultures of strain HS1 <i...
<p>Panel A - Spore coat proteins were extracted analysed by western blotting with anti-CotC antibody...
Coomassie stained SDS-PAGE gel of non-infected control RK13 cells (RK), T. hominis infected RK13 cel...
<p>Western blotting analysis with anti-CotB (Panel A) or anti-IL-2 (Panel B) antibodies. Spore coat ...
Supplemental Figure S3. Densitometry from whole cell lysate (WCL) and extracellular vesicle (EV) wes...
Western blot analysis of HA-tagged IPCS from culture-derived trypomastigotes from WT and IPCS-addbac...
<p>(A) The proteins of interest were analyzed using 50 µg of protein extracts from fibroblast, f-rES...
<p>Western blot analysis of active PC1/3 (A) and PC2 (B) protein expression in control cells and in ...
<p>The strain used for co-IP expressed the respective proteins in suitable amounts (cell extracts); ...
<p>Proteins released in supernatant were revealed with non position-specific antiHis primary antibod...
<p><b>(A)</b> Western blot of acid-solubilized cell lysates (prepared from bacterial cells grown in ...
(A) The detection of C11orf83_6xH constructs by western blotting. McCoy cells were infected with the...
<p>Lines 1–2 show induced Hsp70 class protein, lines 3–4 constitutive Hsp70 and line 5 a 70 kD size....
<p>Spore coat proteins were extracted and analyzed by western blotting with anti-influenza M2 mAb. N...
<p>(A) COS-1 cells transfected with pZeoSV2 empty vector and labeled with <sup>35</sup>S-methionine....
<p><u>Panel A</u>. Western blot analysis of whole cell lysates of in vitro cultures of strain HS1 <i...
<p>Panel A - Spore coat proteins were extracted analysed by western blotting with anti-CotC antibody...
Coomassie stained SDS-PAGE gel of non-infected control RK13 cells (RK), T. hominis infected RK13 cel...
<p>Western blotting analysis with anti-CotB (Panel A) or anti-IL-2 (Panel B) antibodies. Spore coat ...
Supplemental Figure S3. Densitometry from whole cell lysate (WCL) and extracellular vesicle (EV) wes...
Western blot analysis of HA-tagged IPCS from culture-derived trypomastigotes from WT and IPCS-addbac...
<p>(A) The proteins of interest were analyzed using 50 µg of protein extracts from fibroblast, f-rES...
<p>Western blot analysis of active PC1/3 (A) and PC2 (B) protein expression in control cells and in ...
<p>The strain used for co-IP expressed the respective proteins in suitable amounts (cell extracts); ...
<p>Proteins released in supernatant were revealed with non position-specific antiHis primary antibod...
<p><b>(A)</b> Western blot of acid-solubilized cell lysates (prepared from bacterial cells grown in ...