VALIDATION OF DIFFERENT METHODS COMPARING WITH THEIR SPECIFICITY

In literature it is mentioned that different organic solvents (methanol, ethanol, water, acetone and 80% ethanol) are used for the extraction of phytochemical from plants. In this paper we attempted to analyze all these organic solvents and tried to find out that which one is the most suitable solvent for the extraction of phytochemical. One variety of chickpea genotype (JAKI9218) was collected from College of Agriculture, Sehore, Madhya Pradesh. Seed coat were removed and crushed into fine powder. Phytochemicals were isolated using soxhlet apparatus with various solvents. Extract was investigated to find active constituents of the seed extracts by the different phytochemical tests and their anti oxidant activity