BISPHENOL A DETECTION IN PACKAGED DRINKING WATER AND BEVERAGES USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Background: Bisphenol A is characterized as an endocrine disruptor as it interferes with the synthesis of hormones and metabolism resulting in abnormality in the homeostatis of exposed persons. It is used in the production of polycarbonate and epoxy resins which are utilized in the preparation of almost all plastic packaging materials like plastic bottles, cans, food containers, and coating on food containers. Objective: To detect leaching of Bisphenol A in 15 samples of Bottled water and Beverages using High Performance Liquid Chromatography. Methods: Liquid-liquid extraction technique was used for analytical detection of BPA from bottled drinking water and beverages. Results: BPA contamination in Bottled drinking water was calculated through mean concentration for a time period of 30 days as (0.38 ng/ml - 0 day), 8.86 ng/ml (5th day), 17.85 ng/ml (10th day), 30.35 ng/ml (20th day) and 44.48 ng/ml (30th day)). The mean concentration of BPA was observed to be 0.25 to 2.25 ng/ml. Also, the mean concentration of BPA at different temperatures was observed to be 5.96 ng/ml (at 40C), 5.62 ng/ml (at 200C) and 8.80 ng/ml (at 550C). The above results revealed presence of high concentration of BPA in all the samples of bottled drinking water and beverages. Conclusion: The results obtained in the above study depicted considerable amount of BPA leaching from bottled containers into drinking water and beverages. Prolong usage of bottled water and beverages should be avoided to reduce the risk of human exposure to BPA through leaching. Also, it was found that high temperatures resulted in increased BPA leaching