pH biosensor based assay for SLC9B2 using HEK-293 SLC9B2 OE cells

pHluorins are pH‐sensitive genetically encoded fluorescent sensors, that allow measurement of intracellular pH in living cells. In particular, pH-Axxam-Sensor (pHAxensor) is a chimera between a pH-insensitive Red fluorescent protein (RFP) and a pHluorin, pH-sensitive Green fluorescent protein (GFP). At acidic pH, pHluorin is quenched, whereas at physiological pH, pHluorin fluorescence increases. SLC9B2 belongs to the Cation/Proton Antiporter (CPA) superfamily, and it couples inward transport of protons to mediate sodium efflux from the cells. Activation of SLC9B2 leads to pH changes that can be detected by the pH biosensor