Revisited the relationship between vitamin D level and receptors of BsmI- gene polymorphism with the pathogenetic mechanisms of placental dysfunction development

Manasova G. S., Zelinsky A. A., Didenkul N. V., Artemenko V. V., Makshayeva E. T. Revisited the relationship between vitamin D level and receptors of BsmI- gene polymorphism with the pathogenetic mechanisms of placental dysfunction development. Journal of Education, Health and Sport. 2018;8(3):465-475. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.1208109 http://ojs.ukw.edu.pl/index.php/johs/article/view/5388 The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 1223 (26.01.2017). 1223 Journal of Education, Health and Sport eISSN 2391-8306 7 © The Authors 2018; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 01.03.2018. Revised: 10.03.2018. Accepted: 23.03.2018. Revisited the relationship between vitamin D level and receptors of BsmI- gene polymorphism with the pathogenetic mechanisms of placental dysfunction development G. S. Manasova, A. A. Zelinsky, N. V. Didenkul, V. V. Artemenko, E. T. Makshayeva Odessa National Medical University, Ukraine Abstract The role of the calcitriol / vitamin D receptor (VD) endocrine system and the pleiotropic effects of this system in the pathogenetic mechanisms of various diseases development, in particular complications of pregnancy, has attracted researches’ increasing attention in recent years. The aim of the work: to compare the VD-status and frequency of occurrence of polymorphism of the VDR gene (BsmI (A> G, rs1544410) in patients with a physiological course of the gestation process and in patients with placental dysfunction (PD). Materials and methods. 56 pregnant women with PD (the main group) and 40 patients with a physiological pregnancy (control group) were examined. VD status was determined by ELISA at level 25 (OH) D in serum, the frequency of BsmI polymorphism of the VDR gene (rs1544410) by polymerase chain reaction (PCR). Results. The average index of VD (31.40 ± 8.6) ng / ml in patients with PD is significantly lower than in patients with physiological pregnancy (43.54 ± 11.20) ng / ml, (p ≤ 0.05 ). In patients with PD, homozygous carrier for the A-allele was found in 12% of cases, in healthy pregnant women - in 16.7%, (р ≥ 0.05), for the G-allele - in 20% and 47.20%, (р ≤ 0.01) cases, respectively to groups. Heterozygous combination of A / G alleles was noted in 68% of patients with PD and in 36.10% of the control group patients. In pregnant women with BsmI polymorphism of calcitriol gene (genotype A / G) PD was 3.7 times more frequent (68% vs 36.10% : RR = 2.1, CI 1.0-6.6, OR = 3.7, CI 1.1-13.1). Conclusions. Vitamin D insufficiency or deficiency can be one of the reasons of PD formation. In carriers of BsmIgene’s polymorphism encoding VD receptor with genotype A / G, the course of pregnancy is complicated by placental dysfunction 3.7 times more often than in women without this polymorphism. Key words: vitamin D, polymorphism of VDR gene, placental dysfunction