ABSTRACT In vitro experiments on bioaccumulation of heavy metals, chromium, copper, lead and zinc was conducted using water lettuce Pistia stratiotes Engl. (Araceae) with 5, 10 and 20 mg/ 100 ml concentration for each metals for a period of 20 days. The Scanning Electron Microscopy equipped with Energy Dispersive X-ray (SEM-EDX) results revealed the bioaccumulation of lead as high as 37.79% followed by chromium 8.63%, zinc 6.00% and copper 2.37%. There was a change in the pH of the medium, the bioaccumulation of metals were confirmed by FTIR analysis and a unique metabolic studies of heavy metal sorption have been predicted by GC-MS analysis. Keywords: Bioaccumulation, FT-IR, GC-MS, Heavy metals, Pistia stratiotes Engl., SEM-EDX R...