The Effect of 10% Ointment Concentration of Ethyl Acetate Subfraction of Meniran (Phyllanthus niruri L.) Leaves on Excision Wound Healing in White Male Rats

Background: Wounds are skin problems that are often experienced by humans. Effective wound healing requires a complex arrangement of various healing processes that occur continuously. The plant that has been studied to have a role in the wound healing process is the herbal extract of meniran (Phyllanthus niruri L). This plant can be found in almost all parts of Indonesia. However, biomedical evidence related to the effect of giving meniran (Phyllanthus niruri L) leaves ethyl acetate subfraction ointment preparations is still not revealed yet. Methods: This study was carried out using experimental animals, a total of 27 rats were divided into 3 large groups, each group consisted of 9 rats, where group 1 was the control based ointment, group 2 was the comparison group (ointment T®) and group 3 was treated with 10% concentration of meniran leaf ethyl acetate subfraction ointment. Each group was observed and measured for three parameters, namely, percentage of wound healing, epithelialization time, and hydroxyproline levels. Results: From the results of data analysis using one-way (ANOVA) followed by the duncan test (SPSS 23.0) for epithelialization time and hydroxyproline levels, the results showed that 10% concentration of meniran leaf ethyl acetate subfraction ointment, the comparison group (T® ointment) and the control based group with the treatment group on the parameters of epithelialization time and hydroxyproline have levels significant of (p