Fig. 1 in Chemical identification of 18-hydroxycarlactonoic acid as an LjMAX1 product and in planta conversion of its methyl ester to canonical and noncanonical strigolactones in Lotus japonicus

Fig. 1. Detection of carlactonoic acid (CLA), 4-deoxyorobanchol (4DO) and 5- deoxystrigol (5DS) in carlactone (CL) feeding experiment by recombinant Lotus japonicus MAX1 (LjMAX1) and Os900. CL was incubated with yeast microsomes. Yeast microsomes having an empty vector and the expression vector pYeDP60-Os900 were used as a negative and a positive control, respectively. The extracts of the microsomes and authentic standard were analyzed by LCMS/MS. MRM chromatograms of CLA (red: 331.10/69.00, blue: 331.10/ 113.00, m/z in negative mode), 4DO and 5DS (red: 331.15/216.00, blue: 331.15/97.00, green: 331.15/234.00, m/z in positive mode) are shown. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)Published as part of Mori, Narumi, Sado, Aika, Xie, Xiaonan, Yoneyama, Kaori, Asami, Kei, Seto, Yoshiya, Nomura, Takahito, Yamaguchi, Shinjiro, Yoneyama, Koichi & Akiyama, Kohki, 2020, Chemical identification of 18-hydroxycarlactonoic acid as an LjMAX1 product and in planta conversion of its methyl ester to canonical and noncanonical strigolactones in Lotus japonicus, pp. 1-16 in Phytochemistry (112349) 174 on page 3, DOI: 10.1016/j.phytochem.2020.112349, http://zenodo.org/record/829480