During blood stage infection, malaria parasites invade, mature, and replicate within red blood cells (RBCs). This results in a regular growth cycle and an exponential increase in the proportion of malaria infected RBCs, known as parasitemia. We describe a flow cytometry based protocol which utilizes a combination of the DNA dye Hoechst, and the mitochondrial membrane potential dye, JC-1, to identify RBCs which contain parasites and therefore the parasitemia, of in vivo blood samples from Plasmodium chabaudi adami DS infected mice. Using this approach, in combination with fluorescently conjugated antibodies, parasitized RBCs can be distinguished from leukocytes, RBC progenitors, and RBCs containing Howell-Jolly bodies (HJ-RBCs), with a limit...
Background: Malaria pigment (haemozoin, Hz) has been the focus of diverse research efforts. However,...
The invasion of CD71+ reticulocytes by Plasmodium vivax is a crucial yet poorly characterised event....
This protocol describes a methodology for imaging the sequestration of infected erythrocytes of the ...
During blood stage infection, malaria parasites invade, mature, and replicate within red blood cells...
BACKGROUND: Malaria treatments are becoming less effective due to the rapid spread of drug resista...
BACKGROUND Malaria treatments are becoming less effective due to the rapid spread of drug resistant ...
Despite significant technological and conceptual advances over the last century, evaluation of the e...
Despite significant technological and conceptual advances over the last century, evaluation of the e...
Despite significant technological and conceptual advances over the last century, evaluation of the e...
In order to propagate within the mammalian host, malaria parasites must invade red blood cells (RBCs...
Circulating red blood cells consist of young erythrocytes (early and late reticulocytes) and mature ...
In order to propagate within the mammalian host, malaria parasites must invade red blood cells (RBCs...
In order to propagate within the mammalian host, malaria parasites must invade red blood cells (RBCs...
<div><p>In order to propagate within the mammalian host, malaria parasites must invade red blood cel...
The invasion of CD71+ reticulocytes by Plasmodium vivax is a crucial yet poorly characterised event....
Background: Malaria pigment (haemozoin, Hz) has been the focus of diverse research efforts. However,...
The invasion of CD71+ reticulocytes by Plasmodium vivax is a crucial yet poorly characterised event....
This protocol describes a methodology for imaging the sequestration of infected erythrocytes of the ...
During blood stage infection, malaria parasites invade, mature, and replicate within red blood cells...
BACKGROUND: Malaria treatments are becoming less effective due to the rapid spread of drug resista...
BACKGROUND Malaria treatments are becoming less effective due to the rapid spread of drug resistant ...
Despite significant technological and conceptual advances over the last century, evaluation of the e...
Despite significant technological and conceptual advances over the last century, evaluation of the e...
Despite significant technological and conceptual advances over the last century, evaluation of the e...
In order to propagate within the mammalian host, malaria parasites must invade red blood cells (RBCs...
Circulating red blood cells consist of young erythrocytes (early and late reticulocytes) and mature ...
In order to propagate within the mammalian host, malaria parasites must invade red blood cells (RBCs...
In order to propagate within the mammalian host, malaria parasites must invade red blood cells (RBCs...
<div><p>In order to propagate within the mammalian host, malaria parasites must invade red blood cel...
The invasion of CD71+ reticulocytes by Plasmodium vivax is a crucial yet poorly characterised event....
Background: Malaria pigment (haemozoin, Hz) has been the focus of diverse research efforts. However,...
The invasion of CD71+ reticulocytes by Plasmodium vivax is a crucial yet poorly characterised event....
This protocol describes a methodology for imaging the sequestration of infected erythrocytes of the ...