Xu, Jingwei, Peng, Guanzu, Xu, Jinkun, Li, Yi, Tong, Li, Yang, Dong (2021): Probing of the plasticity of the active site in pinene synthase elucidates its potential evolutionary mechanism. Phytochemistry (112573) 181: 1-10, DOI: 10.1016/j.phytochem.2020.112573, URL: http://dx.doi.org/10.1016/j.phytochem.2020.11257
<p>α-Pinene is a natural and active monoterpene, which is widely used as a flavoring agent and in fr...
2.1. Homology modeling of P. lactiflora pinene synthase The crystal structures of pinene synthase...
Pinene is a monoterpenes (C-10) that is produced in a genetically-engineered microbial host for its ...
Fig. 7. The RMSD profiles of the region with high (RMSD H) and low (RMSD L) mobility from the simula...
Fig. 6. S491A mutation increases the rigidity of the active pocket when it binds the pinyl cation. R...
Fig. 1. a, The proposed reaction mechanism for the synthesis of limonene, pinene and sabinene. b, Th...
Fig. 2. Converting pinene synthase to sabinene synthase by mutations on 482 position. Chromatograms ...
Fig. 3. Hybrid quadrupole-orbitrap GC-MS/MS. a shows the chromatogram of terpene products of F482L. ...
Fig. 5. The effect of the S491A mutation. Chromatograms in a, b and c show the GC-MS analysis of ter...
Fig. 4. Conformational differences in the pinyl cation in WT and F482Y as demonstrated by molecular ...
Pinene, a natural active monoterpene, is widely used as a flavoring agent, perfume, medicine, and bi...
Fig. 1. Chemical structures of the pinenes with their two enantiomers: (+)-α-pinene (1), ()-α-pinene...
<p>α-Pinene is a natural and active monoterpene, which is widely used as a flavoring agent and in fr...
<p>α-Pinene is a natural and active monoterpene, which is widely used as a flavoring agent and in fr...
<p>α-Pinene is a natural and active monoterpene, which is widely used as a flavoring agent and in fr...
<p>α-Pinene is a natural and active monoterpene, which is widely used as a flavoring agent and in fr...
2.1. Homology modeling of P. lactiflora pinene synthase The crystal structures of pinene synthase...
Pinene is a monoterpenes (C-10) that is produced in a genetically-engineered microbial host for its ...
Fig. 7. The RMSD profiles of the region with high (RMSD H) and low (RMSD L) mobility from the simula...
Fig. 6. S491A mutation increases the rigidity of the active pocket when it binds the pinyl cation. R...
Fig. 1. a, The proposed reaction mechanism for the synthesis of limonene, pinene and sabinene. b, Th...
Fig. 2. Converting pinene synthase to sabinene synthase by mutations on 482 position. Chromatograms ...
Fig. 3. Hybrid quadrupole-orbitrap GC-MS/MS. a shows the chromatogram of terpene products of F482L. ...
Fig. 5. The effect of the S491A mutation. Chromatograms in a, b and c show the GC-MS analysis of ter...
Fig. 4. Conformational differences in the pinyl cation in WT and F482Y as demonstrated by molecular ...
Pinene, a natural active monoterpene, is widely used as a flavoring agent, perfume, medicine, and bi...
Fig. 1. Chemical structures of the pinenes with their two enantiomers: (+)-α-pinene (1), ()-α-pinene...
<p>α-Pinene is a natural and active monoterpene, which is widely used as a flavoring agent and in fr...
<p>α-Pinene is a natural and active monoterpene, which is widely used as a flavoring agent and in fr...
<p>α-Pinene is a natural and active monoterpene, which is widely used as a flavoring agent and in fr...
<p>α-Pinene is a natural and active monoterpene, which is widely used as a flavoring agent and in fr...
2.1. Homology modeling of P. lactiflora pinene synthase The crystal structures of pinene synthase...
Pinene is a monoterpenes (C-10) that is produced in a genetically-engineered microbial host for its ...