Brucker Maldi-TOF custom database for differentiating Enterococcus faecium clades A1/A2 from B

This custom database has been constructed using MBT explorer software (Bruker ®) and the Maldi Biotyper (Bruker). Protein extraction were performed according to the MSP Creation protocol (V1.1, Bruker ®) and mass spectra were obtained following the Maldi Biotyper protocol (V.2.4, Bruker ®). The resulting spectra were carefully inspected using flexAnalysis software (V3.4, Bruker ®). After elimination of spectra with mass peak deviation > 0.05%, outlier peaks or flatline, the remaining spectra were combined to generate a single mass spectrum for each strain onto the MALDI Biotyper software (V4.1, Bruker ®), with default parameters. The mass spectra were used to generate this new database, available on the MBT Compass RUO software (RevC Version, Bruker ®)