DETERMINATION OF FAT-SOLUBLE VITAMINS CONCENTRATIONS IN BIOLOGICAL SOLUTIONS BY COMPUTER EXPRESS-METHOD

The paper represents the experimental results of elaboration of qualitative method to determine concentrations of vitamins A, D, and E in liquids and biological fluids by aid of computer analysis of visual data. The H.S. Skovoroda’s KNPU Chemistry department staff has been working on a software product for schools under the general name of ―ColorKit‖. The software allows analyzing photo and video files. One of ―ColorKit‖ application directions may be the investigation of liquids and fluids optical densities parameters. Concerning the vitamins solutions, the research primary task was to create a non-polar reagent for each vitamin, so that, the reagent would bind a vitamin and rapidly form a complex of specific color. The intensity of the latter would be proportional to the vitamin concentration, and would be determined by aid of web-camera and the ―ColorKit‖ program. The innovation of reagents preparation was proposed, i.e. saturated solution of SO3 (for vitamin A), or SO3 + N2O5 (for vitamin D and E) in dichloride-ethane. The experimental video analysis according to RGB-system established logarithmic dependences of the solution color on vitamin A concentration. The correlation coefficients were equal to 0.95-0.99. The highest magnitude of R was registered for comparison of vitamin A experimental solution color with methylenic blue solution color, which was used as a colorimetric standard for the computer processing of visual data. The analysis of vitamin E video files according to RGB-system established logarithmic dependences of the solution color on vitamin E concentration with low correlation coefficients. The same analysis according to ―Black-White‖ system displayed the linear correlation between the solutions concentrations and their color with R magnitude equal to 0.998. The experimental vitamin D solutions video analysis to ―Black White‖-system established a linear correlation in the dependence of solution color intensity on the vitamin concentration, with the high R magnitude, which was equal to 0.94. Of high practical application might be the evaluation of vitamin D solutions concentrations dependence upon the samples brightness. Thus, algorithm of processing visual effects of vitamins qualitative colored reactions alterations was established by aid of web-camera CNR-WCAM820 and the ColorKit program. The calibration graphs for the vitamin concentrations determination in solutions were obtained. The elaborated method may be recommended to use at school conditions