Tissue nutrient analyses

Nutrients are essential for plant growth and prosperity and to determine its future nutrient content for human or animal consumption. The nutrients are classified as macronutrients (elements that plants require in large amount such as phosphorus, potassium) and micronutrients (elements required in smaller amounts such as Manganese, Zinc, Boron). Nutrients have multiple functions and more specifically: Phosphorus is an essential nutrient both as a part of plant structure compound, and as a key player in biochemical reactions. It is a vital component of DNA, RNA and ATP, which is an energy unit for vital plant processes. Potassium is taken up in significant amounts by crops and is essential in almost all processes needed to sustain plant growth and reproduction. Nutrients deficiency usually impacts on plant tissue colour and appearance and the symptoms vary depending on the physical location in plant and the deficiency grade. Application and Principle The dried tissue samples were powdered using a blade mill and passed through a 40-mesh sieve. Sub-samples of all dried plant tissue samples were used for chemical analysis to determine P concentrations using dry ashing at 550 °C for 5 h, followed by dissolution of the resulting ash in1 N HCl. The K concentrations in aqueous extracts were determined by flame photometry using a Sherwood Model 420 (Sherwood Scientific, Cambridge, UK).The information presented here has been thoroughly researched and is believed to be accurate and correct. However, the authors cannot be held legally responsible for any errors. There are no warranties, expressed or implied, made with respect to the information provided. The authors will not be liable for any direct, indirect, special, incidental or consequential damages arising out of the use or inability to use the content of this publication