Chlorophyll Assay

Photosynthetic pigments in plants comprise of chlorophylls a and b, which mainly capture light in the antenna complex via photosystem II, with subsequent electron transport. The two chlorophylls differ only at position 3 (ring B): chlorophyll a contains a methyl group at this position, whereas chlorophyll b contains an aldehyde group. Other pigments such as carotenoids are also found in plants and are considered as accessory components in the photosynthetic complex by providing photoprotection and stability of proteins present in the photosystem. To measure the chlorophyll content, we used a modified protocol (Arnon. 1949, Mochizuki et al., 2001). Extraction and determination of chlorophyll and carotenoid content was performed according to the method of Arnon (1949).The information presented here has been thoroughly researched and is believed to be accurate and correct. However, the authors cannot be held legally responsible for any errors. There are no warranties, expressed or implied, made with respect to the information provided. The authors will not be liable for any direct, indirect, special, incidental or consequential damages arising out of the use or inability to use the content of this publication